Effect of Erzhiwan on Invasion and Metastasis of Human Colon Cancer Cells / 中国实验方剂学杂志
Hua-ying WANG; Wen-yan YU; Guo-juan WANG; Hong-ning LIU; Xiang-yan WEI; Peng-yun YING.
Chinese Journal of Experimental Traditional Medical Formulae
; (24): 28-33, 2019.
Artículo
en Zh
| WPRIM | ID: wpr-801961
Objective:
To investigate the effect of Erzhiwan (EZW) on the invasion and metastasis of human colon cancer HCT116 cells.Method:
The abilities of invasion (number of transmembrane cells) and migration (relative width of 48 h scratch) were observed by Transwell assay. Western blot was used to detect vascular endothelial growth factor (VEGF) and E-cadherin protein, respectively.Result:
① Transwell results showed that compared with the blank control group, the number of model piercing cells in each drug group was decreased (PPPPPPPP-1 L-OHP combined with 10% EZW group was lower than that in L-OHP group (PP-1 L-OHP combined with 10% EZW group and L-OHP group increased the expression of E-cadherin protein significantly. Compared with L-OHP group, the expression of E-cadherin protein was the highest (PConclusion:
EZW can inhibit the invasion and metastasis of colon cancer HCT116 cells, and the best effect is achieved after combined with L-OHP. This may be related to the decrease of VEGF protein expression and the increase of E-cadherin protein expression after combination with L-OHP.
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WPRO
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