Objective@#To explore the practice and effect of transparent supervision in the prevention and control of multidrug-resistant organism (MDRO).@*Methods@#Transparent supervision was introduced to intervene into the MDRO management of monitored departments. Such transparent supervision was aided by IT means, and transparent MDRO control indexes. These indexes included hand hygienecompliance rate, qualification rate of ATPdetection of environmental object surface, implementation rate of main MDRO prevention and control measures, MDRO resistance rate, and nosocomial infectionincidence. The data before and after the intervention were compared by Chi square test to evaluation the effect.@*Results@#After intervention, the hand hygienecompliance rate increased from 49.83% to 65.61% (χ2=47.389, P<0.001), and the qualification rate of ATPdetection of environmental object surface increased from 48.50% to 83.33% (χ2=96.115, P<0.001). Meanwhile, such indexes as prescription of patient isolation, placement of isolation identifiers and that of specialized bedside equipments increased significantly(P<0.001). The MDRO detection rate decreased from 75.08% to 63.31% (χ2=28.123, P<0.001), and the incidence of nosocomial infection decreased from 4.77% to 3.47%(χ2=20.303, P<0.001).@*Conclusions@#The practice of transparent supervision could reduce the risk of MDRO transmission at hospitals effectively, proving its importance in ensuring medical quality and patient safety.