OBJECTIVE@#To determine the effect of
indomethacin on high concentration
glucose and
lipopolysaccharide (LPS) induced
fibronectin (FN) and
plasminogen activator inhibitor-1 (
PAI-1)
secretion in cultured
human peritoneal mesothelial
cells.@*
METHODS@#Mesothelial
cells were isolated from
human omental specimens by
trypsin disaggregation and incubated by 2.5%
glucose or LPS together with different concentrations of
indomethacin.
Enzyme-linked immunosorbent assay determined the quantity of FN and
PAI-1 in the cultured supernatants.@*RESULTS@#Compared with the
control group, the levels of FN and
PAI-1 in the cultured supernatants were increased significantly exposuring to high concentration
glucose and LPS (P <0.01). The different concentrations of
indomethacin decreased FN and
PAI-1 secretion in the 2.5%
glucose or the LPS group within 24 h (P < 0.05).@*CONCLUSION@#
Indomethacin can inhibit the synthesis and
secretion of
extracellular matrix in
human peritoneal mesothelial
cells, which may be effective in the
gene therapy for
peritoneal fibrosis.