DNA damage caused by suicide gene therapy system under Tet-On regulation in breast cancer cells / 中南大学学报(医学版)
Hongde LI; Shengguang XIANG; Nan MA; Weixin HU; Zhaojun ZENG.
Journal of Central South University(Medical Sciences)
; (12): 836-843, 2011.
Artículo
en Zh
| WPRIM | ID: wpr-814498
OBJECTIVE@#To determine the effect and molecular mechanism of DNA damage caused by suicide gene therapy system HSV-TK/GCV under Tet-On regulation in human breast cancer cell line MCF-7 infected by recombinant adeno-associated virus (rAAV).@*METHODS@#We used comet assay to detect the effect of HSV-TK/GCV suicide gene regulation system on MCF-7 DNA damage, and analyzed the expression change of relative DNA damage response active genes and proteins with RT-PCR and Western blot.@*RESULTS@#Compared with other control groups, the comet assay showed that MCF-7 cells with HSV-TK/GCV treatment had obvious comet tails, and the expression level of DNA damage response active genes and proteins changed obviously in the HSV-TK/GCV treatment group,such as ATM, p53 and p27,but CyclinE and CDK2 did not change.@*CONCLUSION@#DNA damage on MCF-7 cells is resulted from HSV-TK/GCV in suicide gene therapy system through a p53-dependent signal pathway, causing cell cycle arrest and cell death.
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