Objective To observe the protective effect of
bisoprolol against
hypoxia/reoxygenation
injury of cardiac microvascular
endothelial cells and explore the mechanism.
Methods Left ventricular of cardiac microvascular
endothelial cells (CMECs) were isolated from 8-week-old
male C57BL/6N
mice. CMECs were randomized into four groups
control group, vehicle group,
hypoxia/reoxygenation group (H/R group),
hypoxia/reoxygenation +
bisoprolol group. The level of
cell proliferation,
apoptosis,
superoxide anion, Cleaved
caspase-3 and Nox2 expression were measured in each group. Results Compared with
control group, H/R group had lower
cell proliferation, higher apoptotic level, more
superoxide anion level and the expression of Cleaved
caspase-3 and Nox2 (P < 0.05). Furthermore,
bisoprolol reversed
hypoxia/reoxygenation-induced the decreased
cell proliferation, the increased
apoptosis,
superoxide anion level, Cleaved
caspase-3 and Nox2 expression (P < 0.05). Conclusion
Bisoprolol can protect CMECs against
hypoxia/reoxygenation
injury by reducing the expression of Nox 2 that decreases
oxidative stress.