OBJECTIVE@#To screen, identify, and compare the
serum biomarkers between anovulatory
dysfunctional uterine bleeding (ADUB) and ovulatory
dysfunctional uterine bleeding (ODUB) in Lizu
females.@*
METHODS@#The subjects included 128 ADUB
patients, 63 ODUB
patients, and 93 controls. The
serum and supernate of the subjects' mense were collected and stored at -80 °C until use. Differential
proteins in the sera of three groups were screened using surface-enhanced
laser desorption ionization
time-of-flight
mass spectrometry. The screened
proteins were then identified by tricine-
SDS-PAGE gel and
spectrometry.
Protein expression levels in the menses of ADUB, ODUB, and control subjects were determined using
ELISA, RT-PCR, and
Western blotting. SPSS 14.1 was used for
statistical analysis and chart drawing (α = 0.05).@*RESULTS@#Three differential
protein peaks with peak values of 11.80, 13.59, and 14.68 km/z were screened and identified as
serum amyploid
protein A (SAA),
vascular endothelial growth factor, and
vitamin K epoxide reductase, respectively. The SAA was highly expressed in the menses of ADUB and ODUB
patients but poorly expressed in the controls. The
vascular endothelial growth factor was highly expressed in the menses of ODUB and controls but poorly expressed in ADUB
patients. Meanwhile, the
vitamin K epoxide reductase was highly expressed in the menses of ADUB and control subjects but poorly expressed in ODUB
patients.@*CONCLUSIONS@#The SAA is the common
serum biomarker of ADUB and ODUB. ADUB may be related to
angiogenesis impairment, whereas ODUB may be associated with
blood coagulation disruption.