Objective To investigate the extraction methods for active components from oral ulcerfilm and optimize the determinationmethods of active components dexamethasonesodiumphosphate and metronazole. Methods Different extraction solvents(methanol, water and 70% methanol aqueous) were applied to extract the active components dexamethasonesodiumphosphate and metronazole from oral ulcerfilm, which contents were quantified by a HPLCmethod. Results the extraction solventwater had the best efficacy and more simpler compared to the other two solvents. Clotriazole showed a good linear relationship within 5.014 5-200.5800 μg/ml (r=0.999 8), and the average extraction recovery was (104.23±0.63)%, and for dexamethasonesodiumphosphate, a good linear relationship was obtained in the range of 0.482-16.328 μg/ml (r=0.9999), and the average extraction recovery was (103.97±1.02)%. Conclusion The water extraction method established in this study was simple and efficient, which showed features of simplicity, accuracy and repeatable.