This study is to explore the effect of Qingfei Paidu Decoction(QPD) on the host
metabolism and
gut microbiome of
rats with
metabolomics and 16 S
rDNA sequencing. Based on 16 S
rDNA sequencing of
gut microbiome and
metabolomics(GC-MS and
LC-MS/MS), we systematically studied the
serum metabolites profile and
gut microbiota composition of
rats treated with QPD for continued 5 days by oral gavage. A total of 23 and 43 differential metabolites were identified based on QPD with GC-MS and
LC-MS/MS, respectively. The involved
metabolic pathways of these differential metabolites included
glycerophospholipid metabolism,
linoleic acid metabolism, TCA cycle and
pyruvate metabolism. Meanwhile, we found that QPD significantly regulated the composition of
gut microbiota in
rats, such as enriched Romboutsia, Turicibacter, and
Clostridium_sensu_stricto_1, and decreased norank_f_Lachnospiraceae. Our current study indicated that short-term intervention of QPD could significantly regulate the host
metabolism and
gut microbiota composition of
rats dose-dependently, suggesting that the
clinical efficacy of QPD may be related with the
regulation on host
metabolism and
gut microbiome.