Objective: To explore the effects and potential mechanism of Foshou
Powder on Parkinson’s
disease .
Methods: Reserpine was injected in KM
mice to establish Parkinson’s
disease model, and
hypothermia and akinesia were measured to evaluate the effects of
volatile oil of
Angelica sinensis ,
Ligusticum chuanxiong and combination. The
human monoamine oxidase (hMAO) activity and inhibition mode were measured by
fluorescence spectrophotometry in vitro with
kynuramine as the common substrate of
human monoamine oxidase A (hMAO-A) and
human monoamine oxidase B (hMAO-B). On this basis, the 2 × 4 factorial design was applied to
research the interaction between
Angelica sinensis volatile oil and
Ligusticum chuanxiong
volatile oil .
Results: The antagonistic experiments of
reserpine showed that the application of
Angelica sinensis volatile oil and
Ligusticum chuanxiong
volatile oil significantly improved
hypothermia and akinesia.
Enzyme activity test revealed that
volatile oil of
Angelica sinensis and
Ligusticum chuanxiong had competitive inhibitory effects on hMAO-A and hMAO-B, and the combination of the two substances had significant synergistic effect on the activity of both hMAO.
Conclusion: Angelica sinensis and
Ligusticum chuanxiong in Foshou
Powder have significant synergistic effects in the prevention and
treatment of Parkinson’s
disease , which may be related to the inhibition of
MAO activity.