Using isolated
rat heart preparations, we observed the protective effects of
verapamil cardioplegia on ischemic myocardial
injury. Isolated
rat hearts were subjected to global
ischemia at 25degrees C. Twenty four isolated
Sprague Dawley rat hearts underwent 30 minutes of the retrograde nonworking
perfusion with Krebs-Henseleit
buffer solution followed by 25degrees C
cardioplegic solution(St. Thomas'
Hospital Cardioplegic Solution) for 60 minutes. Before ischemic arrest,
rat hearts were treated with
cold cardioplegic solution in
control group(n=12) and
cold cardioplegic solution with
verapamil(1mg/L) in experimental group(n=12). After 60 minutes of
ischemia,
hemodynamic and biochemical
parameters such as
heart rate, left
ventricular pressure(LVP), +dp/dt max, coronary flow and
creatine phosphokinase(CPK) were measured before giving
cardioplegia and 30 minutes after
reperfusion.
Verapamil group exhibited greater recovery of
heart rate, LVP, +dp/dt max, coronary flow and CPK than
control group(p<0.05).