Study on transport mechanism of brucine and strychnine in MDCK-MDR1 cell monolayer model / 中草药
Ya HU.
Chinese Traditional and Herbal Drugs
; (24): 2876-2883, 2019.
Artículo
en Zh
| WPRIM | ID: wpr-851057
Objective:
To study the bi-direction transport behavior of brucine and strychnine in the MDCK-MDR1 cell monolayer model.Methods:
MTT method was employed to confirm the safe concentration of brucine and strychnine towards MDCK-MDR1 cells. The effects of transport time, drug concentration, and P-glycoprotein inhibitor verapamil on cumulative absorption concentration (Ccum) and apparent permeability coefficient (Papp) of brucine and strychnine in MDCK-MDR1 monolayer cells were studied.Results:
The Papp value of brucine and strychnine was larger than 1 × 10-5 cm/s and the ratio of Papp(BL→AP) vs Papp(AP→BL) was less than 2. Brucine/ strychnine combined with verapamil decreased the ratio of Papp(BL→AP) vs Papp(AP→BL).Conclusion:
The absorption of brucine and strychnine in MDCK-MDR1 cell monolayer model was well and the passive transference was its main intestinal absorption mechanism. The P-gp inhibitor verapamil has a significant inhibitory effect on brucine and strychnine absorption. Brucine and strychnine may be a substrate of P-glycoprotein.
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