Extraction of phenylethanoid glycoside from Cistanche tubulosa and purification technology by macroporous resin / 中草药
Yu-Jie WANG.
Chinese Traditional and Herbal Drugs
; (24): 2344-2348, 2014.
Artículo
en Zh
| WPRIM | ID: wpr-854835
Objective:
To optimize the extraction and purification technology of phenylethanoid glycoside from Cistanche tubulosa.Methods:
Orthogonal design L9(34) was employed to optimize the extraction conditions by taking the extraction yield of echinacoside and acteoside as indexes. The absorption-desorption characteristics of seven macroporous resins were evaluated, then the best purification conditions were optimized.Results:
The optimal extraction conditions were as follows The air-dried stems of C. tubulosa were powdered and extracted twice with 12-fold 50% ethanol for 1 h each time, temperature was 70℃; The supernatant was concentrated to 5-fold weight of the stems of C. tubulosa. The concentrated liquid was subjected to macroporous resin (HPD750) column and then eluted with deionezed water (3 BV) and 30% ethanol (4 BV), respectively. The 30% ethanol fraction was evaporated under vacuum to give the phenylethanoid glycoside-rich C. tubulosa stem extract. The purity of phenylethanoid glycoside was above 75%.Conclusion:
The optimized extraction and purification process is stable, efficient, and suitable for industrial production.
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WPRO
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