The appearance time of all chromatographic peaks of Zuojin Pills was within 8 min and 9 common chromatographic peaks were obtained. The linear is stable and peak form is better. The linear relationship and resolution of the five alkaloids are nice. The similarity of the fingerprints exceed 0.98; The contents of the five alkaloids are much more different, the content of the five alkaloids in the HCl-methanol sample is the most, however, the least is the water extraction sample.
Conclusion:
This study shows that the UPLCmethod is convenient with quick analysis rate. On account of indicated component quantitative analysis, it could be used to synthetically evaluate the extraction method. HCl-methanol (1100) extraction method is the best one.