Effect of MG132 on acute myeloid leukemia cell proliferation and apoptosis / 中国药理学通报
Xiao-Hui ZHENG.
Chinese Pharmacological Bulletin
; (12): 327-334, 2019.
Artículo
en Zh
| WPRIM | ID: wpr-857334
Aim:
To investigate the effect of proteasome inhibitor MG132 on the proliferation and apoptosis of acute myeloid leukemia cells.Methods:
qRT-PCR was used to detect the expression of ADRM1 mRNA in nine blood tumor cell lines. The expression of ADRM1 in HL60 cells was interfered by shRNA; HL60 cells before and after ADRM1 interfered were treated with different MG132 concentrations for 24 h; Then, the cell proliferation and viability were measured with CCK-8 by microplate reader. Meanwhile, the expressions of ADRM1 and UCH37 protein were detected by Western blot. Apoptosis of HL60 and NB4 cells treated with different MG132 concentrations was analyzed by flow cytometry.Results:
ADRM1 mRNA was up-regulated in blood tumor cell lines. ADRM1 shRNA and scrambled shRNA HL60 cells were successfully constructed. Cell proliferation and viability were inhibited by AD-RM1 shRNA interference or decreased with the increase of MG132 concentration; meanwhile, ADRM1 and UCH37 protein expressions were down-regulated. The apoptosis of HL60 and NB4 cells increased with the increase of MG132 concentrations. The apoptotic effect of MG132 on HL60 cells was stronger than that of NB4 cells.Conclusions:
ADRM1 mRNA is overexpressed in blood tumor cell lines; ADRM1 down-regulation induces UCH37 protein decrease and cell proliferation inhibition. MG132 induces AML cell apoptosis and restrains the proliferation and viability through down-regulating the expression of ADRM1 and UCH37 protein. The apoptotic effect of MG132 on different types of AML cells exists individual differences.
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