Discussion on the analytical method of sodium lactate in compound sodium lactate and glucose injection in chinese pharmacopoeia / 中国药学杂志
Xiong-Liang XU.
Chinese Pharmaceutical Journal
; (24): 2081-2085, 2015.
Artículo
en Zh
| WPRIM | ID: wpr-859288
OBJECTIVE:
To establish a RP-HPLC method for the determination of sodium lactate in compound sodium lactate and glucose injection and provide reference for revision of method recorded in Chinese Pharmacopoeia.METHODS:
The separation was performed on a Shim-pack VP-ODS C18 analytical column (4.6 mm×250 mm, 5 μm), and the mobile phase was formic acid-dicyclo-hexylamine-water (11998, V/V) at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 210 nm and the column temperature was maintained at 40℃. The loading volume was 20 μL. The RP-HPLC method and the cation exchange resin method adopted by Chinese Pharmacopoeia were both used to determine the content of sodium lactate in compound sodium lactate and glucose injection, and the results were compared.RESULTS:
The linear range of the RP-HPLC method was 0.51-6.07 mg·mL-1 (r=0.9999). The average recovery was 99.47% (RSD=0.75%, n=9).CONCLUSION:
The established method of RP-HPLC can be used for the determination of sodium lactate in compound sodium lactate and glucose injection.
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