Effect of Tibetan medicine Oxytropis falcata on cell apoptosis and expression of Bcl-2 and Bax protein in SMMC-7721 cells / 中国药学杂志
Guang-Ming YANG.
Chinese Pharmaceutical Journal
; (24): 2113-2116, 2014.
Artículo
en Zh
| WPRIM | ID: wpr-860112
OBJECTIVE:
To probe the influence of Oxytropis falcata on apoptosis and expression of Bcl-2 and Bax proteins in SMMC-7721 cells.METHODS:
Annexin V-FITC/ PI assay was applied to detect apoptosis in SMMC-7721 cells after being treated with TFOF (total flavonoids of O. falcata). Changes of Bcl-2 and Bax expression were investigated by Western blot.RESULTS:
Cell apoptosis was induced significantly in SMMC-7721 cells when subjected to TFOF. Western blot showed that Bcl-2 expression was downregulated. Although Bax expression was also downregulated slightly comparing with that of control, the ratio of Bcl-2/Bax decreased significantly after TFOF treatment.CONCLUSION:
The results suggest that TFOF of O. falcata may be a pro-apoptotic agent for hepatic cancer cells via its modulation of Bcl-2/Bax balance.
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