Objective: To investigate the isolation characteristics and the
antibiotic resistance of
Streptococcus pneumoniae in
hospitalized children from the Bao′an District of Shenzhen for many consecutive years, and to provide evidence for the assessment as well as clinical prevention and
treatment of
Streptococcus pneumoniae infection .
Methods: The isolation rate and sample source of
Streptococcus pneumoniae isolates as well as age composition of
hospitalized children in Bao′an
Maternal and Child Health Hospital of Shenzhen city from January 2012 to December 2018 were retrospectively analyzed.The susceptibility of the isolates to
antibiotics was tested by disk
diffusion method and E-test
method .
Results: (1) From 2012 to 2018, a total of 90 807 specimens were retrospectively summarized, 9 193
strains of
Streptococcus pneumoniae were isolated, with an isolation rate of 10.1%, which was the first pathogen; 98.68% (9 072/9 193 cases) from
respiratory tract samples and 97.20% from
sputum samples.Among 107
strains isolated from aseptic
body fluid specimens, 97
strains were
blood specimens.
Streptococcus pneumoniae was the most common
bacteria with positive
culture in aseptic
body fluids .(2) The isolation rate of
Streptococcus pneumoniae was 10.7% in
children under 2 years old, 10.2% in
children between 2 and 5 years old and 4.1% in
children over 5 years old.About 97% of
Streptococcus pneumoniae isolates came from
children under 5 years old.(3) According to breakpoints of non-
meningitis strains , the
sensitivity rate of parenteral
Penicillin was 93.18%, the
mediation rate was 6.79%, the resistance rate was only 0.03%, the lowest
drug concentration to inhibit the
growth of 50%
bacteria (MIC 50) value was 0.50 and the lowest
drug concentration to inhibit the
growth of 90%
bacteria (MIC 90) value was 2.00; the
sensitivity rate of Ceftria-xone was 76.55%, the
mediation rate was 20.18%, the resistance rate was 3.26%, the MIC 50 value was 1.00 and the MIC 90 value was 2.00; when interpreted by the breakpoint of
meningitis strains , the resistance rate of extra-gastrointestinal
Penicillin reached 77.22%, and the
mediation rate of
Ceftriaxone increased to 30.48% and the
drug resistance rate reached 18.45%.The
drug resistance rates of
Erythromycin ,
Clindamycin ,
Tetracycline and Compound
neomycin were all over 85.00%.The resistance rates against
Levofloxacin and
Chloramphenicol were 0.16% and 7.85% respectively.No
Vancomycin -resistant
Streptococcus pneumoniae was found.
Conclusions: Streptococcus pneumoniae is the first bacterial pathogen causing
respiratory tract and invasive
infection in
hospitalized children under 5 years old in this area.
Penicillin is still the preferred
drug for non-
meningitis pneumococcal infection , but the
drug resistance rate is high in
meningitis patients .