Methyl thiazolyl tetrazolim (MTT) colorimetric method was used to detect the proliferation inhibition rate of K562/VCR cellsin vitro after intervention with Brucea javanica oil emulsion.
Results:
With the increasing concentration of Brucea javanica oil emulsion (125,250,500,750,1 000 mg/L), the inhibition ratio to K562 cells continued to increase ( P<0.05), and with the increasing duration of Brucea javanica oil emulsion(24,48,72 h), the inhibition ratio to K562 cells continued to increase ( P<0.05). With the increasing concentration of Brucea javanica oil emulsion(500,750,1 000,1 500,2 000 mg/L), the inhibition ratio of K562/VCR cells continued to increase ( P<0.05), and with the increasing action time of Brucea javanica oil emulsion (24,48,72 h), the inhibition ratio of K562/VCR cells continued to increase ( P<0.05). With the increasing concentration of Brucea javanica oil emulsion(blank control group , Brucea javanica oil emulsion group for 500,750,1 000,1 500 mg/L), the G 0/G 1 ratio in K562/VCR cell cycle continued to increase, while the S ratio and G 2/M ratio continued to decrease ( P<0.05). After injection of Brucea javanica oil emulsion, ADM concentration (fluorescence intensity) in the cells increased significantly(161.4 ± 10.9 vs. 95.9 ± 8.1)( P<0.05).