Fracture-related infections are a major problem that plagues many orthopedic surgeons.As their diagnosis is often doubtful, treatmenttime-consuming, healing prolonged and expenditure huge, they se-riously damage the physical and mental health of patients.In view of the differences in injury mechanism, injury severity, bacterial origin and onset time in patients with fracture-related infection, it is particularly important to standardize the clinical collection of specimens to increase positive rate of bacterial culture and detection rate of pathogenic bacteria.This article expounds on preparation, timing, location, type, quantity and post-processing in specimens’ collection, hoping that a fine understanding of standardized procedures in specimens’ collection may better serve clinical treatment.