Brain Material Basis and Relevant Mechanism of Acute Stroke Treated with Rhei Radix et Rhizoma Based on Homotherapy for Heteropathy Using Proteomics / 中国实验方剂学杂志
To explore the material basis and mechanism of acute stroke treated with Rhei Radix et Rhizoma based on Homotherapy for Heteropathy using the analysis of proteomics and bioinformatics.
Method:
A total of 60 male Sprague-Dawley(SD)rats were randomly divided into ischemic stroke(IS) sham-operation group (Sham1), IS model group (IS), IS+ Rhei Radix et Rhizoma treatment group (DH1),ICH sham-operation group (Sham2), intracerebral hemorrhage(ICH) model group (ICH), and ICH + Rhei Radix et Rhizoma treatment group (DH2), with 10 rats in each group. After cerebral perfusion, the braintissues were quantified by proteomic analysis, and differentially expressed proteins (DEPs) were identified. Specimens of IS, Sham1, and DH1 groups were collected at 24 hours, while those of ICH, Sham2, and DH2 groups were collected at 48 hours. The common DEPs were analyzed by bioinformatics, and the relevant DEPs were verified by Western blot.