Objective: To select
volatile oils from 16 species of
plants (
Cymbopogon citratus,
Pelargonium graveolens,
Pinus tabulieformis,
Litsea cubeba,
Mentha haplocalyx,
Zingiber officinale ,
Syzygium aromaticum ,
Curcuma longa ,
Zanthoxylum bungeanum,
Cinnamomum cassia ,
Ocimum basilicum ,
Rosmarinus officinalis ,
Zanthoxylum schinifolium,
Zanthoxylum armatum,
Illicium verum ,
Myristica fragrans ) that have good inhibitory effect on the
growth of
Aspergillus flavus .
Method: Aspergillus flavus was isolated from the surface of Platycladi
Semen medicinal materials by plate
culture method . The
volatile oils of 16
plants were extracted by
steam distillation . The colony diameter of
Aspergillus flavus was determined by
fumigation of filter
paper , and the effect of
volatile oils on the
growth of
Aspergillus flavus was systematically studied.
Result: Aspergillus flavus was successfully isolated from Platycladi
Semen by means of morphological, microscopic and
DNA barcoding identification
methods , the bacteriostatic rates of the above 16 kinds of
volatile oils against
Aspergillus flavus were 2.93%, 0.05%, 0.37%, 76.07%, 0.34%, 0.15%, 50.05%, 8.51%, 1.43%, 58.20%, 0.07%, 2.60%, 8.73%, 100.00%, 52.62%, 0.07%, respectively.
Conclusion: The
volatile oils of 16
plants all have different degrees of antibacterial activities for
Aspergillus flavus , and
volatile oils of
Zanthoxylum armatum,
Litsea cubeba and
Cinnamomum cassia have good inhibitory effect. This study can provide a theoretical basis for the prevention and control of
Aspergillus flavus in the
growth and storage of Platycladi
Semen , and provide a basis for further
research on
plant volatile oil as bacteriostatic agents in the storage process of
traditional Chinese medicine .