Objective: To study the mechanism of Taoren Chengqitang in regulating intestinal myoelectric activity and microenvironment
homeostasis in intestinal
sepsis rats based on high mobility group
protein 1(
HMGB1 )/
Toll-like receptor 4 (TLR4)/nuclear factor -κB(NF-κB) pathway.
Method: The 60 SD
rats were randomly divided into sham operation group, model group,
glycyrrhizic acid (
HMGB1 inhibitor, 0.03 g·kg-1) group, Taoren Chengqitang group (10 g·kg-1),
glycyrrhizic acid +Taoren Chengqitang group (0.03 g·kg-1+10 g·kg-1), with 12
rats in each group. Except the sham operation group, the other groups established intestinal
sepsis rat models, each group was treated with
medicine ,
hematoxylin -
eosin (HE)
staining was used to detect the histopathological changes of small
intestinal mucosa in
rats of each group, the changes of mucosal thickness and villus height were compared, the levels of
secretory immunoglobulin A (
sIgA ),
diamine oxidase (DAO) and
D-lactic acid in
intestinal mucosa of
rats were detected by kit, the intestinal myoelectrical activity of
rats in each group was measured, the slow
wave frequency and amplitude of small intestinal
smooth muscle were compared, the
intestinal flora of
rats in each group was detected, the contents of E. coli,
Bifidobacterium and
Lactobacillus were compared, and the expressions of
HMGB1 /TLR4/NF-κB pathway
proteins HMGB1 , TLR4, MyD88 and NF-κB p65 in small intestinal
tissues were detected by
Western blot .
Result: Compared with sham operated group, the villus height, mucosal thickness,
sIgA content, slow
wave frequency and amplitude of
smooth muscle ,
Bifidobacterium and
Lactobacillus contents in
intestinal mucosa of model group
rats were significantly decreased, and
serum DAO and
D-lactic acid levels, intestinal E. coli content, intestinal
tissue HMGB1 , TLR4, MyD88 and NF-κB p65
proteins were significantly increased (P<0.05). Compared with the model group, the villus height, mucosal thickness,
sIgA content, slow
wave frequency and amplitude of
smooth muscle ,
Bifidobacterium and
Lactobacillus contents in
intestinal mucosa of the Taoren Chengqitang group,
glycyrrhizic acid group, and
glycyrrhizic acid + Taoren Chengqitang group were significantly increased, and
serum DAO and
D-lactic acid levels, intestinal E. coli content, intestinal
tissue HMGB1 , TLR4, MyD88 and NF-κB p65
proteins were significantly decreased (P<0.05). Compared with the Taoren Chengqitang group and the
glycyrrhizic acid group, the villus height, mucosal thickness,
sIgA content, slow
wave frequency and amplitude of
smooth muscle ,
Bifidobacterium and
Lactobacillus contents in
intestinal mucosa of
glycyrrhizic acid +Taoren Chengqitang group were significantly increased, and
serum DAO and
D-lactic acid levels, intestinal E. coli content, intestinal
tissue HMGB1 , TLR4, MyD88 and NF-κB p65
proteins were significantly decreased, the differences were statistically significant (P<0.05).
Conclusions: Taoren Chengqitang can alleviate intestinal mucosal
injury , regulate intestinal myoelectrical activity and microenvironment
homeostasis , restore intestinal function and maintain
flora balance in intestinal
sepsis rats , which may be achieved by down-regulating
HMGB1 /TLR4/NF-κB pathway.