To establish a mouse model of diminished ovarian reserve (DOR) induced by tripterygium wilfordii polyglycosides (TWP), and to explore the therapeutic effect of Dingkundan (DKD) on DOR, so as to provide scientific basis for its clinical application.
Compared with the blank group, most of the mice in model group had disordered estrous cycle, uterine and ovarian indexes decreased (P<0.05), serum FSH increased (P<0.05), LH was on an upward trajectory, E2 was on a downward trend, and the number of growth follicles and corpus luteum decreased and the number of atresia follicles increased(P<0.05). Compared with the model group, half of the mice in DKD group resumed regular estrous cycle, however, the estrous cycles of mice in estradiol valerate group were stagnated during estrous period. In medium-dose, high-dose DKD group and estradiol valerate group, the uterine and ovarian indexes of the mice were increased, the serum FSH value decreased (P<0.05) and serum LH was on a downward trend, high-dose DKD group and estradiol valerate group increased the levels of serum E2 (P<0.05). In DKD group, the number of growth follicles and corpus luteum were increased and the number of atresia follicles were reduced (P<0.05), with the best effect at medium dosage. And in estradiol valerate group, the number of primitive follicles, sinusoidal follicles and corpus luteum were increased (P<0.05), but the number of atresia follicles had no difference to the model group.