BACKGROUND@#Osimertinib is approved by
Food and Drug Administration for
patients with advanced
non-small cell lung cancer carrying EGFR-T790M
mutations. Osimertinib
therapy was missed in many
patients who were unable to perform
biopsy due to occult lesion progression or weak body. In this study. We
hope that some
proteins associated with predicting EGFR-T790M resistance could be screened from the
serum to provide help for clinical medication. The aim of this study is to explore the
protein associated with EGFR-T790M
drug resistance gene and provide help for clinical medication.@*
METHODS@#In this study, 36
patients with advanced
lung adenocarcinoma treated by
gefitinib were included. After the
disease progression of the
patients,
biopsy was performed. 18
patients in the EGFR-T790M
mutation group and 18
patients in the non-EGFR-T790M
mutation group were detected by the
ARMS method.
Serum of
patients with
drug resistance was collected, and
proteins related to EGFR-T790M resistance were screened by isotopic marker relative and absolute quantitative marker combined with two-dimensional
liquid chromatography tandem mass spectrometry proteomics technology.@*RESULTS@#Seventeen different
proteins were screened out, including 6 up-regulated
proteins and 11 down-regulated
proteins associated with EGFR-T790M
gene mutation, which were mainly involved in 31
biological processes, 7
cell components and 26 molecular functions. Twelve enrichment pathways were identified, among which the highest enrichment index was the coagulation cascade pathway.@*CONCLUSIONS@#Seventeen
proteins associated with EGFR-T790M resistance were found, and
proteins involved in the coagulation cascade pathway are expected to be
biomarkers associated with predicting EGFR-T790M resistance
mutations.