Alpinia officinarum Hance of the
Chinese traditional herb for the
treatment of
emesis ,
abdominal pain and
diarrhea has been used to counteract
gastric disease induced by
indomethacin in
rats without obvious side effects.However,the
role of
herb-drug interaction between
indomethacin and A.officinarum based on pharmacokinetic,
tissue distribution and excretion still remains unknown.In this study,an ultra-fast liquid-
tandem mass spectrometry (UFLC-MS/MS)
method was developed for simultaneous determina-tion of
indomethacin and its three metabolites,O-desmethylindomethacin(ODI),deschlor-obenzoylindomethacin(NDI)and
indomethacin acyl-β-D-
glucuronide (IDAβG)by
oral administration of
indomethacin solution with and without the ethanolic extract of A.officinarum and applied to comparative pharmacokinetic,
tissue distribution and excretion studies.Our results clarified that
oral administration of A.officinarum produced significant alterations in the pharmacokinetic
parameters of
indomethacin .And the pharmacokinetic interaction between
indomethacin and A.officinarum reduced the systemic exposure of
indomethacin and increased its elimination.
Tissue distribution results demonstrated that co-
administration of A.Officinarum could not reduce the accumulation of indo-
methacin in the target
tissue of the
stomach ,but could accelerate the excretions of
indomethacin and its three metabolites including ODI,NDI and IDAβG in the
bile and
feces of
rats in the excretion study.Therefore,A.Officinarum might have a gastrointestinal protective effect through the interaction
role with
indomethacin based on the
pharmacokinetics and excretion in
rats .