Thirty-two cases of brucellosis group, including 6 females and 26 males, aged (50.312 ± 5.035) years; 30 cases of control group, including 4 females and 26 males, aged (49.994 ± 5.098) years. There were no significant differences in sex ratio and age between the two groups (χ 2=0.336, t = 1.744, P > 0.05). There were no significant differences in the frequency distribution of G, A allele and GG, GA, and AA genotypes of rs7579 locus of Sepp1 gene between brucellosis group and control group (χ 2=0.263, 0.942, P > 0.05). In stratified analysis, there was a statistically significant difference in the frequency distribution of genotypes between femalebrucellosis group and control group (GG 0/6, GA 2/6, AA 4/6; GG 4/4, GA 0/4, AA 0/4, P < 0.05). And the level of Sepp1 protein in brucellosis group was significantly lower than that in control group (13.71±0.32, 19.26±0.69, t = 20.316, P < 0.05), low level of Sepp1 was a risk factor for brucellosis ( OR = 1.512, 95% CI 1.290 - 1.687).