Modified technique of ureter implantation for urinary tract reconstruction in murine renal transplantation model / 中华器官移植杂志
Yi YU; Zirong BI; Dong WANG; Jun LI; Qifeng JIANG; Shicong YANG; Qian FU; Longshan LIU; Changxi WANG.
Chinese Journal of Organ Transplantation
; (12): 48-51, 2021.
Artículo
en Zh
| WPRIM | ID: wpr-885311
Objective:
To explore the application value of modified technique of ureter implantation in murine renal transplantation.Methods:
Thirty left donor kidneys from BALB/c mice was transplanted into syngeneic mice. Cuff technique was applied for anastomosing kidney artery and vein. The procedure of ureter-bladder anastomoses shifted from implication-fixation-embedding to fixation-implication-embedding. Operative duration, recipient survival rate and complications were recorded.Results:
Time for separating vessels, perfusion and excision of donor graft was (25±3) min, (10±6) s for warm ischemia and (25±5) min for cold ischemia. Time for separating recipient vessels was (12±5) min, (7±1) min for arterial anastomosis, (7±1) min for venous anastomosis, (13±2) min for ureter-bladder anastomosis, (5±1) min for right kidney excision and (5±1) min for abdominal closure. Operative duration was(77±3)min. Twenty-six recipients survived over 3 months. The successful operative rate was 86.7%.Conclusions:
With a shorter learning curve, modified technique of ureter implantation is easier and faster so as to reduce the postoperative incidence of urinary tract complications during murine renal transplantation.
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WPRO
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