Background@#This study investigated the longitudinal associations between the degrees of positive and negative spillover in work–life balance (WLB) at baseline and reports of depressive mood at a 2-year follow-up in Korean womenemployees. @*Methods@#We used a panel study design data of 1386 womenemployeeswho participated in the Korean Longitudinal Survey of Women and Families in both 2014 and 2016. Depressive mood was measured using the “10-item Center for Epidemiologic StudiesDepression Scale.” Associations between the positive and negative spillover in WLB at baseline and reports of new incidence of depressive mood at 2-year follow-up were explored using a multivariate logistic regression model. @*Results@#Negative spillover in WLB at baseline showed a significant linear association with reports of depressive mood at 2-yearfollow-up after adjusting for age, education level, marital status, number of children, and positive spillover (P = 0.014). The highest scoring group in negative spillover (fourth quartile) showed a significant higher odds ratio of 1.95 compared with the lowest scoring group (first quartile; P = 0.036). @*Conclusion@#Positive spillover in WLB showed a U-shaped association with depression. The degrees of positive and negative spillover in WLB among Korean womenemployees at baseline were associated with new incidence of depressive mood within 2 years. To prevent depression of femaleworkers, more discrete and differentiated policies on how to maintain healthy WLB are required.