Compared with control group, the levels of the maximum ascending rate of left ventricular pressure(+dp/dt<sub>max</sub>), the maximum descending rate of left ventricular pressure(-dp/dt<sub>max</sub>) and the left ventricular systolic blood pressure(LVSP) were decreased significantly(<italic>P</italic><0.05),but the level of the left ventricular end diastolic blood pressure (LVEDP) was significantly increased(<italic>P</italic><0.05). The expressions of GRP78, p-PERK, PERK, ATF6, XBP1, CHOP, Bax and apoptosis index were increased significantly (<italic>P</italic><0.05,<italic>P</italic><0.01), while Bcl-2/Bax and Bcl-2 were decreased significantly(<italic>P</italic><0.01) in model group. Compared with the model group, the levels of -dp/dt<sub>max</sub> and Bcl-2 were increased significantly (<italic>P</italic><0.05),while the level of the apoptosis index was decreased significantly(<italic>P</italic><0.01) in low-dose Wenxin granule group. In high-dose Wenxin granule group, the levels of +dp/dt<sub>max</sub> and -dp/dt<sub>max</sub> were increased significantly(<italic>P</italic><0.05,<italic>P</italic><0.01), the levels of endoplasmic reticulum stress pathway proteinGRP78, p-PERK, PERK, ATF6, XBP1,apoptosis-related protein CHOP, Bax and apoptosis index were decreased significantly(<italic>P</italic><0.01), but Bcl-2 and Bcl-2/Bax were increased significantly (<italic>P</italic><0.01).
Conclusion:
Wenxin granule has effect in inhibiting excessive endoplasmic reticulum stress, reducing myocardial cellapoptosis and improving cardiac hemodynamics. Its molecular mechanism may be related to decrease the levels of GRP78, PERK, p-PERK, ATF6, XBP1, CHOP, Bax and increasing the expression of Bcl-2.