We identified and analyzed the components and chemical constituents of
hawthorn leaves of
Crataegus pinnatifida Bge. (wild) and C. pinnatifida Bge. var major N. E. Br (cultivated) by using ultra
high-performance liquid chromatography and quadrupole
time-of-flight
mass spectrometry (UHPLC-Q-TOF/MSE) combined with UNIFI
data analysis platform and multivariate
statistics. Fifty-eight
chemical compounds were identified, including
organic acids,
flavonoids, triterpenoic
acids,
monoterpenes and
sesquiterpenoids; among them,
terpenoid content was the most abundant.
Principal component analysis (
PCA) and orthogonal partial
least squares discriminant analysis (OPLS-DA) were used to identify the differential components of
hawthorn leaves from two sources. The results show that there are differences in the chemical compositions of the two sources, including 24
flavonoids and
terpenoids (including
monoterpenoids,
sesquiterpenoids and
triterpenoid acids). The types of
flavonoids (such as
rutin, vitexin-2''-O-rhamnoside, isovitexin-2''-O-rhamnoside, hyperoside,
quercetin-3-O-β-D-
glucoside) and
terpenoids (crataegolic
acid, corosolic
acid and
ursolic acid) in C. pinnatifida were more varied than those found in C. pinnatifida Bge. var major N. E. Br, and their contents were relatively higher. This study provides a comprehensively
analysis of the different chemical components of
hawthorn leaves from two sources listed in the
Chinese Pharmacopoeia, and provides a basis for the selection of raw materials and the potential development and utilization of
hawthorn leaves.