Purpose@#
Adenosine A2A receptor agonist
polydeoxyribonucleotide (PDRN) possesses an anti-inflammatory effect and suppress apoptotic
cell death in several disorders. In this current study, the effect of PDRN on
inflammation and
apoptosis in
rats with
Achilles tendon injury was investigated. @*
Methods@#von Frey filament test and plantar test were conducted for the
determination of
pain threshold.
Analysis of histological alterations was conducted by
hematoxylin and
eosin staining.
Immunohistochemistry for cleaved
caspase-3-positive
cells and cleaved
caspase-9-positive
cells was done.
Enzyme-linked
immunoassay was used to detect the concentrations of
tumor necrosis factor (TNF)-α,
interleukin (IL)-6, and cyclic
adenosine-3’,5’-monophosphate (cAMP).
Western blot was conducted to detect the
protein levels of
cAMP response element-binding protein (CREB),
protein kinase A (PKA), Bcl-2-associated X (Bax), and
B-cell lymphoma 2 (Bcl-2). @*Results@#PDRN
treatment relieved
mechanical allodynia and alleviated
thermal hyperalgesia after
Achilles tendon injury. TNF-α and
IL-6 concentrations were decreased by PDRN application. PDRN
injection significantly enhanced cAMP concentration and phosphorylated CREB versus CREB ratio, showing cAMP-PKA-CREB pathway was activated by PDRN application. PDRN
treatment inhibited percentages of cleaved
caspase-3-positive
cells and
caspase-9-posiive
cells and the suppressed Bax versus Bcl-2 ratio in
Achilles tendon injury rats. @*Conclusions@#PDRN is probably believed to have a good effect on
pain and
inflammation in the urogenital organs. PDRN may be used as a new
treatment for
Achilles tendon injury.