Selection of potential
disease-specific
biomarkers from
saliva or
epithelial tissues through
next generation sequencing (NGS)-based
protein studies has recently become possible. The
early diagnosis of
oral squamous cell carcinoma (OSCC) has been difficult, if not impossible, until now due to the lack of an effective OSCC
biomarker and efficient molecular validation
method. The aim of this study was to summarize the advances in the application of NGS in
cancer research and to propose potential proteomic and genomic
saliva biomarkers for NGS-based study in OSCC
screening and
diagnosis programs. We have reviewed four categories including definitions and use of NGS, salivary
biomarkers and OSCC, current
biomarkers using the NGSbased
technique, and potential salivary
biomarker candidates in OSCC using NGS