ObjectiveTo compare the effect of different
solvent extracts of
spore powder and fruiting body of Lasiosphaera Calvatia on
fibroblasts and
wound healing of full-thickness
skin defect, in order to screen the optimal medication part of Lasiosphaera Calvatia. MethodThe effect of
water extract and alcohol extract of
spore powder and fruiting body on
cell proliferation and
cell migration of
mouse skin fibroblasts (MSF) were examined
in vitro.
Cell proliferation and activity test (
CCK-8)
method was used for
cell proliferation, scratch assay was used for
cell migration,
flow cytometry was conducted to explore
cell cycle,
enzyme-linked immunosorbent assay (
ELISA) was used to determine the
production of
collagen Ⅰ and Ⅲ. At the same
time, a full-thickness
skin defect
wound model was established to investigate the
therapeutic effect of different
solvent extracts of
spore powder. Ultraviolet-visible
spectrophotometry was used to
measure the contents of index components in different
solvent extracts. ResultThe
water extract of
spore powder and fruiting body had certain cytotoxicity, while the alcohol extract could promote proliferation, migration and
production of
collagen Ⅰ and Ⅲ of MSF, and the effect of
spore powder was significantly higher than that of fruiting body. When the concentration was 10 mg·L-1, the
cell proliferation rate of alcohol extract of
spore powder was as high as (159.22±15.95)%, and could promote MSF from the G0/
G1 phase to
S phase and G2/
M phase with an increased proliferation index. The alcohol extract also promoted the migration of
fibroblasts, secreted
collagen Ⅰ and Ⅲ. On in vivo model, the alcohol extract of
spore powder significantly accelerated
wound healing on
mice, effectively promoted the complete epithelialization of
wound tissue, and generated new
collagen fiber. The results of
determination showed that the contents of
polyphenols and
flavonoids in the alcohol extract were higher than the alcohol extract of fruiting body. ConclusionThe alcohol extract of
spore powder in Lasiosphaera Calvatia has active components in the
treatment of
wounds with good development prospect, and the medicinal components may be
polyphenols and
flavonoids.