ObjectiveTo explore the effect of Buyang Huanwutang (BHD) on
rehabilitation of
ischemic stroke (IS) by
cell membrane solid-phase
chromatography and
network pharmacology . MethodCell
membrane solid-phase
chromatography was performed to screen the specific binding components of BHD with hippocampal
neurons . Targets of the specific components were retrieved based on PubChem and PharmMapper and those of IS were searched from
Online Mendelian Inheritance in Man (
OMIM ) and GeneCards. Then, the
protein -
protein interaction (PPI) network was constructed with STRING and Cytoscape 3.7.1, followed by
Gene Ontology (GO) term enrichment and Kyoto
Encyclopedia of
Genes and
Genomes (KEGG) pathway enrichment of the hub
genes in the PPI network. Thereby, the mechanism of BHD in promoting IS
rehabilitation was clarified. ResultA total of 13 specific components were identified. The hub
genes were mainly involved in the
biological processes of
regulation of
cell proliferation ,
protein phosphorylation ,
hypoxia response, and
angiogenesis , and the pathways of Forkhead box O (FoxO) signaling pathway,
adenosine 5'-monophosphate (AMP)-activated
protein kinase (AMPK) signaling pathway,
nuclear factor kappa B (NF-κB) signaling pathway, and
apoptosis pathway. ConclusionBHD may promote the recovery of IS by regulating FoxO, AMPK, NF-κB, and
apoptosis pathways.