OBJECTIVE@#To determine the optimum staining condition of teasolutions on bovine incisorsin vitro, by comparing the color stability of tooth surface of different concentrations of teasolutions and methods on bovine incisorsin vitro.@*METHODS@#Twenty bovine incisors with color surface A1 were chosen, then randomly divided into 4 groups (n=10). Group 1 soaked with 2% teasolution continuously for 6 days; group 2 soaked with 2% teasolution for 6 days, but changed fresh teasolution everyday; group 3 soaked with 1% teasolution continuously for 6 days; group 4 soaked with 1% teasolution for 6 days but fresh teasolution changed every day. After 6 days of staining, the surface color (Δ E value) of all the samples were measured with crystal eye. After brushing 30 times with toothbrushes, the color of bovine incisors were measured again. Then the samples were soaked in artificial saliva at 37 ° C, and Δ E value was measured for 14 days.@*RESULTS@#After staining for 6 days, the Δ E values of the 2% teasolution groups were better than those of the 1% groups (20.21 vs. 16.44, 24.09 vs. 19.22, P<0.05); the groups with the same teasolution concentration, a better result was observed for the group soaked with daily fresh teasolution than for the group that experienced continuous staining (24.09 vs. 20.21, 19.22 vs. 16.44, P<0.05). Groups 1 and 2 were selected for subsequent brushing experiments. The color of both groups became lighter after brushing, and a better result was observed for the continuous staining group than for the group stained in daily fresh solution (3.06 vs. 9.51, P<0.05). The samples with better coloring effect soaked with 2% teasolution continuously for 6 days were put into artificial saliva for 14 days. There was not any significant change in coloring at the end of the first two days (1.51 vs. 1.51, P>0.05), and the color was visibly lighter after the third day (1.51 vs. 5.89, P<0.05), and no further significant change was observed until the 14th day (5.81 vs.5.89, P>0.05), which was darker coloring than that of the pre-staining group.@*CONCLUSION@#Continuous staining on bovine incisors with 2% teasolution with subsequent soaking in artificial saliva resulted in consistent coloring from day 3 to day 14, and this method could be used as an ideal model for teethstainingin vitro.