Objective : To study the effect of
temperature and
light intensity on photosynthetic
fluorescence parameters,
volatile oil content, and
growth of
Atractylodes lancea and provide reference for the rational selection of cultivation
environment for A. lancea. MethodWe determined the photosynthetic indexes (such as net photosynthetic rate,
water use efficiency, and carboxylation rate),
light response curve, CO2 response curve,
fluorescence parameters, and the content of four
volatile oils in A. lancea under two
temperature treatments (32 °C and 22 °C) and two
light treatments (full
light and shade). ResultThe net photosynthetic rate and
water use efficiency of A. lancea under high
temperature + strong
light were significantly higher than those under high
temperature + weak
light and low
temperature + strong
light. The
ability of A. lancea to use weak
light at low
temperature was the strongest, while the utilization rate of weak
light under strong
light significantly reduced. The photosynthetic rate of A. lancea at low
temperature was more susceptible to
light intensity and CO2 concentration than that at high
temperature. The maximum photosynthetic rate and apparent quantum
efficiency under weak
light were significantly higher than those under strong
light. The photoreaction
efficiency at high
temperature was higher than that at low
temperature. The total amount of
volatile oil in A. lancea treated with high
temperature + weak
light was the highest, reaching 4.582%. Compared with high
temperature + strong
light, high
temperature + weak
light significantly increased the content of hinesol and β-eudesmol in A. lancea by 91.7% and 35.7%, respectively, and low
temperature + strong
light significantly increased the content of hinesol by 87.5%. The content of β-eudesmol in low
temperature + weak
light treatment was significantly lower than that in high
temperature + weak
light treatment. ConclusionTThe
growth of A. lancea was affected by the interaction between
temperature and
light. The
light and
temperature conditions required for the accumulation of
volatile oil were not consistent with those suitable for the
growth and development of A. lancea. A. lancea responded to the changes of
light and
temperature conditions by regulating the synthesis and accumulation of
volatile oil.