Objective To study the influence of
curcumin on chemosensitivity of
nephroblastoma cells.
Methods Human nephroblastoma cells line SK-NEP-1 was transplanted to the
nude mice subcutaneously to establish the implantation
tumor model of
human nephroblastoma cells. A total of 30
tumor-bearing
mice were divided into three groups of ten randomly. The routine
chemotherapy group was given
vincristine (0.05 mg/mL·0.2 mL/d) and
actinomycin D (15 ng/mL·0.2 mL/d) combined
chemotherapy regime. The
curcumin chemotherapy group was given the same combined
chemotherapy regimens and
curcumin (30 mg/kg/d) by
intraperitoneal injection. The
control group was given
normal saline (NS) of the same volume by
intraperitoneal injection. Continuous
administration would be kept for 4 weeks and 3 days a week. The volumetric changes of every group were recorded. The
serum of every group in different
time was collected and the
VEGF content was detected by
ELISA. All
mice were cercrificed and the
tumor tissues were stripped and weighed after 4 week's
treatment. The
tumor inhibition rate was calculated. The
cell proliferation activity and
apoptosis rate were detected by MTT and
flow cytometry method. All data were statistically analyzed by SPSS 19.0. Results The
tumor volume,
serum VEGF content,
tumor inhibition rate,
cell proliferation activity and
apoptosis rate of routine
chemotherapy group and
curcumin chemotherapy group had significant differences comparing with the
control group (P < 0.05) after 4-week's
treatment. The
cancer growth of
curcumin chemotherapy group was obviously decreased and even tended to shrink comparing with routine
chemotherapy group (χ