Esophageal squamous cell carcinoma (ESCC) is a kind of malignant
tumor with high
incidence and
mortality in the
digestive system . The aim of this study is to explore the function of lnc-ABCA12-3 in the development of ESCC and its unique mechanisms. RT-PCR was applied to detect
gene transcription levels in
tissues or
cell lines like TE-1, EC9706, and HEEC
cells .
Western blot was conducted to identify
protein expression levels of mitochondrial
apoptosis and
toll-like receptor 4 (TLR4)uclear factor kappa-B (NF-κB) signaling pathway.
CCK-8 and EdU assays were carried out to
measure cell proliferation , and
cell apoptosis was examined by
flow cytometry .
ELISA was used for checking the changes in
glycolysis -related
indicators .Lnc-ABCA12-3 was highly expressed in ESCC
tissues and
cells , which preferred it to be a candidate target. The TE-1 and EC9706
cells proliferation and
glycolysis were obviously inhibited with the
downregulation of lnc-ABCA12-3, while
apoptosis was promoted. TLR4 activator could largely reverse the
apoptosis acceleration and relieved the proliferation and
glycolysis suppression caused by lnc-ABCA12-3
downregulation . Moreover, the effect of lnc-ABCA12-3 on ESCC
cells was actualized by activating the TLR4/NF-κB signaling pathway under the
mediation of exosome. Taken together, the lnc-ABCA12-3 could promote the proliferation and
glycolysis of ESCC, while repressing its
apoptosis probably by regulating the TLR4/NF-κB signaling pathway under the
mediation of exosome.