ObjectiveTo explore the application value of high-
risk HPV-
DNA detection combined with TCT in cervical lesion
screening and follow-up. MethodsThis study was conducted from February 2019 to February 2021. During this period, 241 subjects
who were the
patients referred from the primary
community hospitals to the center for re-examination of cervical lesions. Of which 80 were
patients with
cervical intraepithelial neoplasia. Liquid-based
cytology (TCT)
technology was used to examine the cervical cytological morphology. The second-generation
high-throughput sequencing technology and
time-of-flight
mass spectrometry technology, independently developed by BGI, were used to screen for HPV-
DNA typing. At the same
time,
biopsy sampling was carried out, and the final pathological
diagnosis was made. TCT
detection and combined HPV-
DNA detection were performed on 80
patients to compare the relationship between TCT alone and TCT/HPV-
DNA combined test for the
diagnosis and
prognosis of CIN Ⅱ and CIN Ⅲ. Follow-up was conducted, and the
HPV infection and TCT were re-tested. ResultsAmong the 80 cases of
cervical intraepithelial neoplasia, 41 cases (51.25%) were between 35 and 44 years old, higher than the other
age groups (P<0.001). Compared with either TCT or HPV-
DNA, TCT combined with HPV-
DNA had obvious advantages in
sensitivity,
specificity,
positive predictive value, and
negative predictive value (P<0.05). The
recurrence rate in CIN Ⅱ and CIN Ⅲ groups was 9.52% and 16.67% respectively after 18 months of the follow-up. ConclusionTCT
detection plays a positive
role in cervical pre-
cancer screening. By combining high-
risk HPV-
DNA typing and TCT
detection, the
detection values of
sensitivity and specificity are significantly higher, which can improve the accuracy of cervical lesion
screening and is of great significance for the follow-up
work.