@#ObjectiveTo investigate the effects of
nitric oxide synthase (NOS) genetic
transfection on the intimal
hyperplasia of venous
autografts. MethodsThe external
jugular veins were autografted into
abdominal aorta arteries in 20
Wistar rats, which were divided evenly into experimental or
control groups. The transplanted
veins of experimental group were immersed in the
adenovirus-mediated eNOS
gene solution for 15 minutes just before anastomosis. The transplanted vascular samples were taken out 2 weeks after operation. The intimal thickness(IH), degree of restenosis(DR), expression of
PCNA and NOS
mRNA were determined with
histology and transcription
polymerase chain reaction (
PCR). ResultsThe IH, DR and
PCNA decreased, while the expression of eNOS
mRNA increased comparing with
control group(P<0.01). ConclusionTransfection of NOS
gene can inhibit the intimal
hyperplasia of venous
autografts.