The immunomodulatory effect of Saposhnikoviae Radix
polysaccharide(SRP) was evaluated based on the
zebrafish mo-del, and its mechanism was explored by
transcriptome sequencing and real-
time fluorescence-based quantitative
PCR(RT-qPCR). The immune-compromised model was induced by
navelbine in the
immunofluorescence-labeled transgenic
zebrafish Tg(lyz DsRed), and the effect of SRP on the density and distribution of
macrophages in
zebrafish was evaluated. The effect of SRP on the numbers of
macrophages and
neutrophils in wild-type AB
zebrafish was detected by
neutral red and
Sudan black B
staining. The content of NO in
zebrafish was detected by DAF-FM DA
fluorescence probe. The content of
IL-1β and
IL-6 in
zebrafish was detected by
ELISA. The differentially expressed
genes(DEGs) of
zebrafish in the blank
control group, the model group, and the SRP
treatment group were analyzed by
transcriptome sequencing. The immune
regulation mechanism was analyzed by
Gene Ontology(GO)and Kyoto
Encyclopedia of
Genes and
Genomes(KEGG)enrichment, and the expression levels of key
genes were verified by RT-qPCR. The results showed that SRP could significantly increase the density of immune
cells in
zebrafish, increase the number of
macrophages and
neutrophils, and reduce the content of NO,
IL-1β, and
IL-6 in immune-compromised
zebrafish. The results of
transcriptome sequencing
analysis showed that SRP could
affect the expression level of immune-related
genes on
Toll-like receptor pathway and
herpes simplex infection pathway to
affect the release of
downstream cytokines and
interferon, thereby completing the activation process of
T cells and playing a
role in regulating the immune activity of the body.