OBJECTIVE@#To identify sarcosaphagous flies and their larvae, pupa.@*METHODS@#Sarcosaphagous flies and their larvae, pupas were collected from humancorpses and their surroundings in the Weifang city. A 304 bp region in COI gene was analyzed by mtDNA sequencing.@*RESULTS@#The studied region showed no sequence divergence within same species and significant difference were found between different species in all samples.@*CONCLUSION@#It is a practical approach to identify these Sarcosaphagous flies and their larvae, pupas by sequence analysis of the 304bp region of the COI in mtDNA.