Background@#
Epigenetic studies, particularly
research on
microRNA (
miRNA), have flourished. The abnormal expression of
miRNA contributes to the development of
hematologic malignancies.miR-765 has been reported to inhibit
cell proliferation by downregulating proteolipid
protein 2 (PLP2), which causes
apoptosis. We investigated miR-765 dysregulation in
myelodysplastic syndromes (MDS). @*
Methods@#We compared the expression profiles of miR-765 in 65
patients with MDS and 11 controls.
Cell proliferation and
apoptosis assays were performed to determine the
in vitro effects of miR-765 on
leukemia cells transfected with the miR-765 mimic.
Reverse transcription quantitative
PCR (RT-qPCR) and
western blotting were performed to examine the targets of miR-765. @*Results@#We found that miR-765 levels were upregulated 10.2-fold in
patients with MDS compared to controls. In refractory
cytopenia with multilineage dysplasia, the percentage of
patients with elevated miR-765 levels was significantly higher than in other forms of MDS.Experiments with
leukemia cells revealed that
transfection with a miR-765 mimic inhibited
cell proliferation and induced
apoptosis. RT-qPCR and
western blotting demonstrated that the target of miR-765 was PLP2. @*Conclusion@#These findings imply that
upregulation of miR-765 induces
apoptosis via
downregulation of PLP2 and may have a
role in MDS pathogenesis.