Why is it important to understand the nature and chemistry of tannins to exploit their potential as nutraceuticals?

Tannins comprise a large group of polyphenols that can differ widely in chemical composition and molecular weight. The use of tannins dates back to antiquity, but it is only in recent years that their potential use as nutraceuticals associated with the human diet is beginning to be exploited. Although the biological effects of these phytocomplexes have been studied for many years, there are still several open questions regarding their chemistry and biotransformation. The vastness of the molecules that make up the class of tannins has made their characterisation, as well as their nomenclature and classification, a daunting task. This review has been written with the aim of bringing order to the chemistry of tannins by including aspects that are sometimes still overlooked or should be updated with new research in order to understand the potential of these phytocomplexes as active ingredients or technological components for nutraceutical products. Future trends in tannin research should address many questions that are still open, such as determining the exact biosynthetic pathways of all classes of tannins, the actual biological effects determined by the interaction of tannins with other molecules, their metabolization, and the best extraction methods, but with a view to market requirements.

Washed hydrochar from spent coffee grounds: A second generation of coffee residues. Evaluation as organic amendment.

Spent coffee grounds (SCG) hydrochar is a second-generation solid waste obtained by hydrothermal carbonization (HTC). Two washed hydrochars from SCG (175 and 185 °C; 12 MPa of N2) were tested as organic amendments of an agricultural soil (Cambic Calcisol), at doses of 1 and 2.5%, in an in vitro assay using Lactuca sativa as a crop plant. The washed hydrochars differ from the SCG in organic carbon (OC) (56 vs 47%), C/N ratio (29 vs 24), polyphenols (186 vs 77 mg GAE/g), pH (4.1 vs 5.8), assimilable P (186 vs 1274 ppm) and K (32 vs 2475 ppm). The particles of washed hydrochars have a lower size and a more porous structure than SCG particles. Higher HTC temperatures generate greater differences with SCG. The effects on the soil of washed hydrochars are similar to SCG, regarding OC, total N, C/N ratio, available K and P contents. The influence of SCG and washed hydrochars on lettuces is also similar: both give rise to plant growth inhibition and increase in the contents of Ca, Mg, Cu, Fe and Mn. Nevertheless, washed hydrochars seem to be more effective in the mobilization of elements in the soil than SCG, which could be attributed to their higher content of polyphenols. For example: Ca (139 mg/100 g with SCG and 160 mg/100 g with hydrochar) and Fe (0.742 mg/100 g with SCG and 1.45 mg/100 g with hydrochar). Therefore, it can be concluded that SCG hydrochars could be used as organic amendments with similar limitations and advantages to SCG.

Characterization of rums sold in Spain through their absorption spectra, furans, phenolic compounds and total antioxidant capacity.

A total of 42 different rums currently marketed in Spain were analyzed to study the effect of aging time and manufacturing steps (filtration, addition of additives or spices, solera aging method, use of different types of aging barrels) on several parameters: color, non-enzymatic browning, antioxidant capacity and phenolic profile. Different analytical techniques to obtain a broader descriptions of the samples were employed: absorption and UV-vis spectrophotometry, antioxidant capacity (DPPH, FRAP, ABTS methods), total phenols and HPLC to detect individual phenolic and furanic compounds. Results showed that spectrophotometric techniques could potentially be used to detect adulteration and frauds, as well as to differentiate rums by aging time. Those rums aged longer, especially those aged in oak barrels that had previously contained Bourbon or wine, showed higher phenolic content, antioxidant capacity and concentration of furanic compounds. Filtration results in the loss of antioxidant compounds while adding spices increases their concentration in rums.

Bioactivity of food melanoidins is mediated by gut microbiota.

Melanoidins are an important component of the human diet (average consumption 10 g/day), which escape gastrointestinal digestion and are fermented by the gut microbiota. In this study melanoidins from different food sources (coffee, bread, beer, balsamic vinegar, sweet wine, biscuit, chocolate, and breakfast cereals) were submitted to an in vitro digestion and fermentation process, and their bioactivity was assessed. Some melanoidins were extensively used by gut microbes, increasing production of short chain fatty acids (mainly acetate and lactate) and favoring growth of the beneficial genera Bifidobacterium (bread crust, pilsner and black beers, chocolate and sweet wine melanoidins) and Faecalibacterium (biscuit melanoidins). Quantification of individual phenolic compounds after in vitro fermentation allowed their identification as microbial metabolites or phenolics released from the melanoidins backbone (specially pyrogallol, 2-(3,4-dihydroxyphenyl)acetic and 3-(3,4-dihydroxyphenyl)propionic acids). Our results also showed that antioxidant capacity of melanoidins is affected by gut microbiota fermentation.

Effect of in vitro digestion-fermentation on green and roasted coffee bioactivity: The role of the gut microbiota.

Coffee is one of the most consumed beverages and has been linked to health in different studies. However, green and roasted coffees have different chemical composition and therefore their health properties might differ as well. Here, we study the effect of in vitro digestion-fermentation on the antioxidant capacity, phenolic profile, production of short-chain fatty acids (SCFAs), and gut microbiota community structure of green and roasted coffee brews. Roasted coffees showed higher antioxidant capacity than green coffees, with the highest level achieved in fermented samples. Polyphenol profile was similar between green and roasted coffees in regular coffee brews and the digested fraction, but very different after fermentation. Production of SCFAs was higher after fermentation of green coffee brews. Fermentation of coffee brews by human gut microbiota led to different community structure between green and roasted coffees. All these data suggest that green and roasted coffees behave as different types of food.