Prevalence of Haemogregarina stepanowi and Assessment of Some Risk Factors in Mauremys rivulata (Valenciennes, 1833) Freshwater Turtles (Testudines: Geoemydidae)

Objective: The Balkan terrapin, Mauremys rivulata, is a freshwater turtle. This reptile is exposed to many environmental pollutants and some infectious agents, including Haemogregarina stepanowi parasite. This study was conducted to determine the microscopic prevalence of haemogregarine infection in M. rivulata caught in three different localities (Bozcaada, Gökçeada, and Dardanos) in Çanakkale province of Turkey, and assessment of some risk factors. Methods: Twenty-four blood samples were collected, thin blood smears were prepared, and the presence of haemogregarine parasites microscopically was screened. Water samples were also taken from the habitats, and these samples were analyzed physiochemically and microbiologically. Results: Morphological identification was made by detecting the sausage-shaped intra-cytoplasmic developmental stages of H. stepanowi, and thirteen of twenty-four turtles (54.2%) were found to be infected. The prevalence of H. stepanowi was detected as the highest (90.0%) in the Gökçeada district, where the water pollution is higher than in the other localities. A statistically significant relationship was observed between the distribution of the infection and the gender of the turtles, the temperature of the water, the number of faecal coliforms in water and the amount of dissolved oxygen in the water. A statistically significant difference was found between the localities in terms of the prevalence of H. stepanowi infection, and the infection was primarily detected in the Gökçeada district. Conclusion: This study has significance in providing information regarding haemoparasitic diseases of freshwater turtle, M. rivulata, in Turkey.

Determination of the effects of environmental pollution on the Balkan terrapin, Mauremys rivulata (Valenciennes, 1833).

The effects of environmental pollution on three populations of the Balkan terrapin [Mauremys rivulata (Valenciennes, 1833)] from the Bozcaada, Gökçeada and Dardanos regions were evaluated. The morphological parameters of Balkan terrapins collected on each site were measured and blood samples were taken for haematological analysis and micronucleus detection. The physicochemical, microbiological and microelement analyses of the water samples from each region were conducted by standard methods. The highest red blood cell, white blood cell and mean corpuscular haemoglobin concentration values were seen in the samples from Gökçeada. The highest haemoglobin value was found in the samples originating from Bozcaada, whereas the highest haematocrit and mean corpuscular volume values were found in the animals from Dardanos. Based on the microbiological analysis of the water samples, the most polluted site was Gökçeada. The microelement contents of the water and blood samples were different at the three sites, the lowest being in the Gökçeada area. It was revealed that the percentage of red blood cell micronuclei and other nucleus abnormalities in the M. rivulata blood samples was the lowest also in the animals living in the region of Gökçeada.