Effect of Local Rosuvastatin Administration on Calvarial Bone Defects.

The purpose of this study is to investigate the potential of the local administration of different doses of rosuvastatin (RSV) on autogenous grafted critical-sized cortical bone defects. Twenty-four rats were divided into 3 groups: Group C (control), Group RSV-0.1, and Group RSV-1. A 5-mm diameter critical-size defect was created in the calvarium of each animal. In Group C, the defect was filled by autogenous graft and sterile saline-treated absorbable collagen sponge (ACS) was applied. Defects in the experimental groups (groups RSV-0.1 and RSV-1) were grafted by autogenous graft and ACS with saline solution containing 0.1- and 1-mg RSV were applied. All animals were euthanized at 28 days after operation. Stereologic and micro-computed tomography (µCT) analyses were performed. New bone area and connective tissue volumes were measured. Stereologic analysis showed that the difference between group RSV-1 with a mean bone formation of 1.79 ±â€Š0.06 mm and groups RSV-0.1 and control (C) was statistically significant (P ≤ 0.05) with a mean bone formation of 1.29 ±â€Š0.28 mm and 1.08 ±â€Š0.12 mm, respectively. Connective tissue volume was also significantly higher in 1-mg RSV applicated group. Micro-CT results were similar with stereologic analyses. Local administered 1-mg RSV enhances bone regeneration in critical-size calvarial rat defects filled with autogenous graft.

Radiological and Stereological Evaluation of the Effect of Rifampin on Bone Healing in Critical-Size Defects.

OBJECTIVES: This study aimed to investigate the effect of rifampin with autogenous bone on bone regeneration in critical-size defects in the calvaria of rats. MATERIALS AND METHODS: In total, 40 rats were divided into 4 groups and a 5-mm diameter of calvarial defect was made in each rat's calvarium. Control group (C), bone defects were irrigated with sterile saline; rifampin group (R), bone defects were irrigated with rifampin. In the autogenous graft group (Ag), the autogenous graft was contaminated with saliva, and the defects were filled with an autogenous graft. In the autogenous graft + rifampin group (Ag+R), the autogenous graft was contaminated with saliva and was decontaminated with rifampin, and the defects were filled with the autogenous graft. The animals were killed at 4weeks. Bone formation was assessed by micro-computed tomography scanning and stereological analyses. RESULTS: The mean new bone volume was the greatest in the Ag/rifampin group (1.73 ±â€Š0.17), followed by the Ag group (1.50 ±â€Š0.05) (statistically significant difference at P < 0.05). The new bone volume was the lowest in the control group (1.05 ±â€Š0.09); however, no difference was observed compared with the rifampin group (1.08 ±â€Š0.07) (P > 0.05). CONCLUSION: This study, despite its limitations, showed that rifampin with autogenous bone increased bone regeneration in rats with critical-size defects.