ABSTRACT
The effects and interactions of incubation time and chick preplacement holding time on mortality at placement, utilization of yolk sac, crop filling rate, early feeding-drinking behavior, and broiler live performance were investigated. Ross 308 broiler hatching eggs from a 39-week-old flock were set in two identical setters in a commercial hatchery, with the setting time 12 h earlier in one machine. At the end of incubation, chicks were removed from the hatchers at the same time. Thus, the incubation times were either 504 h (normal incubation time (NIT) treatment) or 516 h (longer incubation time (LIT) treatment). After the pull time, chicks from each incubation time group were subjected to either 6, 24, 48, 60, or 72 h preplacement holding times. At placement, chicks were given access to feed and water. In total, 19,200 chicks were randomly assigned to a total of 10 subtreatment groups (2 incubation times × 5 preplacement holding times). Therefore, a total of 1920 chicks were used in each subtreatment group for the grow-out period in a commercial broiler house. For the first week of the experiment, 160 randomly selected as-hatched (not sexed) chicks were placed in 12 replicate floor pens (120 total pens). From the second week of age onward, chicks from two pens were combined into six replicate pens, with 320 chicks per replicate (60 total pens). An interaction was found between incubation time and preplacement holding time for residual yolk sac (RYS) weight (g, %) (p < 0.001). RYS weight was greater at pull time and at 6 and 24 h of preplacement holding in the NIT treatment compared to the LIT treatment, while differences were no longer evident at 48-72 h. The lowest percentage of chicks with full crops and eating activity was observed in the shortest preplacement holding time (6 h) group at 3 h after placement. As expected, the initial BW at placement clearly decreased with increasing duration after the pull time (p < 0.05), with the highest and lowest weights found in the 6 and 72 h holding time treatments, respectively. This BW difference was still evident at 35 d after placement and chicks held for the longest period after the pull time (72 h) showed the lowest BW (p < 0.001). However, there was no significant difference between the 6 and 60 h preplacement holding times. Mortality during the first 7 d after placement increased only when the preplacement holding time was extended to 72 h (p = 0.031). Similarly to the 7 d results, chicks held for 72 h exhibited higher 0-35 day mortality compared to those held for 6 or 24 h (p = 0.028). Neither BW nor mortality was affected by incubation time treatment at 35 d after placement (p > 0.05). It can be concluded that there were no significant differences in average BW and mortality, up to and including a 60 h holding time under thermal comfort conditions, but a 72 h preplacement holding time resulted in final BW and mortality being negatively affected. In addition, LIT tended to have a beneficial effect on BW and mortality compared to NIT when the preplacement holding time was shorter (6-24 h) but had a negative effect for extended holding times (48-72 h).
ABSTRACT
This study investigated the effect of the egg cooling profile after oviposition on blastoderm development, embryonic mortality, hatchability, and hatch time of broiler hatching eggs from young and old breeder flocks. Hatching eggs were obtained from commercial Ross 308 broiler breeders at 28 wk (young) and 64 wk (old) of age. A total of 3,150 eggs laid within a 15-min period were collected and randomly assigned to 2 temperature-controlled chambers in both flocks. The eggshell temperature (EST) was cooled to 24°C either within 6 h (control) or 45 min (rapid). After the EST reached 16°C in the chambers in all groups, eggs were transported to the commercial hatchery. Eggs were stored for 6 d at 16°C and 75% relative humidity. The development of the blastoderm in sampled eggs (25 embryos in each batch) was determined immediately after egg collection and before transport to the hatchery (after cooling) on a farm in each flock. At each flock age, there were 5 replicate trays of 150 eggs per egg cooling treatment set in a single commercial incubator. The results showed that the embryonic developmental stage was retarded by rapid cooling and by the younger flock. A flock age × cooling rate interaction was observed for fertile hatchability and early and late embryonic mortality (P < 0.001). In the young flock eggs, the fertile hatchability was significantly lower in the rapid than in the control cooling treatment (88.7 vs. 92.8%) due to higher early and late embryonic mortality, whereas rapid cooling reduced early embryonic mortality (P < 0.01) and numerically increased the fertile hatchability (88.7 vs. 87.2%) in the old flock eggs. Hatch time was affected by the cooling treatment. The average hatch time was delayed by 3 h by rapid cooling (486.2 vs. 489.2 h) after oviposition compared with the control. This study showed that cooling the EST to 24°C within 45 min (rapid cooling) compared to 6 h (control) after laying retarded the blastoderm developmental stage and hatch time of eggs from both young and old broiler breeder flocks. This was apparently detrimental for the young flock as indicated by the higher early and late embryonic mortality but beneficial for the old flock due to the lower early embryonic mortality. The differences in hatchability between young and old flock eggs resulting from a rapid cooling rate might depend on the differences in embryonic development at oviposition.
Subject(s)
Chickens , Oviposition , Female , Animals , Ovum , Fertility , Embryonic DevelopmentABSTRACT
The effects of the storage period and prewarming temperature on embryonic mortality, hatchability, and synchronous hatching of broiler eggs were investigated. Eggs were obtained from commercial flocks of Ross 308 broiler breeders at 27 and 28 wk of age for trials 1 and 2, respectively. In both trials, 2,400 eggs were stored for 4 d (short) or 11 d (long) at 18°C (64.4°F) and 75% RH and were randomly assigned to 2 groups at either a prewarming temperature of 26.1°C (79°F, low) or 29.4°C (85°F, high) for 8 h before setting. The eggs were transferred from setters to hatching baskets at 444 h (18.5 d) of incubation. The hatched chicks were counted at 6-h intervals between 468 h and 516 h of incubation and categorized as early, middle, or late hatching. The eggs stored for 4 d hatched earlier than the eggs stored for 11 d (P < 0.05). An increased prewarming temperature (29.4°C) resulted in a 1.0-h shorter incubation duration, but this difference was not significant (P = 0.064). An interaction between the storage period and prewarming temperature was observed for middle- and late-hatched chicks (P < 0.05). No interactions between the storage period and prewarming temperature were observed for hatchability of fertile eggs or embryonic mortality; however, a significant interaction was found between the storage period and prewarming temperature on the second-quality chick percentage (P < 0.05). The eggs stored for 11 d had a significantly reduced hatchability of fertile eggs owing to increased embryonic mortality than short-stored eggs (P < 0.05). The interaction effect indicated that eggs held for 8 h with prewarming at 29.4°C after 11 d of storage had more middle- and fewer late-hatched chicks and improved chick quality than those that received the 26.1°C prewarming treatment (P < 0.05), but no significant difference was found among the prewarming treatments for eggs stored for 4 d. This study demonstrated that prolonged egg storage resulted in reduced hatchability, increased incubation duration, and an asynchronous hatching time. Moreover, increasing the prewarming temperature could be used to promote uniformity among embryos through synchronous hatching, thus improving broiler flock uniformity and performance of the prolonged stored eggs.
