ABSTRACT
Micronucleus (MN) induction in erythrocytes of multiple intestinal neoplasia (Min) mice with heterozygous Apc mutation was measured after s.c. injections of acrylamide, glycidamide, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and colchicine, and compared with wild-type (wt) mice. Since Apc influences microtubule dynamics, we wanted to test whether Min-mice were more sensitive to the production of MN than wild-type mice. We also examined the effect of pre-treatment with cytosine beta-D-arabinofuranoside (Ara C) and hydroxyurea, which inhibit ligation of DNA strand breaks in the repair of DNA adducts. All compounds induced a significant increase in MN in both strains of mice with the following potencies: acrylamideSubject(s)
Acrylamide/toxicity
, Adenomatous Polyposis Coli Protein/genetics
, Erythrocytes/drug effects
, Imidazoles/toxicity
, Micronuclei, Chromosome-Defective/chemically induced
, Mutagens/toxicity
, Adenomatous Polyposis Coli/genetics
, Animals
, Colchicine/toxicity
, Cytarabine/pharmacology
, DNA Adducts/metabolism
, Epoxy Compounds/toxicity
, Female
, Flow Cytometry
, Hydroxyurea/pharmacology
, Injections, Subcutaneous
, Male
, Mice
, Mice, Inbred C57BL
, Mice, Mutant Strains
, Micronucleus Tests