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Biotechnol Lett ; 36(12): 2551-8, 2014 Dec.
Article in English | MEDLINE | ID: mdl-25214215

ABSTRACT

The development of the microalgal industry requires advances in every aspect of microalgal biotechnology. In this regard, the availability of genetic engineering tools for industrially-promising species is key. As Scenedesmus almeriensis has promise for industrial use, we describe here an Agrobacterium-based methodology that allows stable genetic transformation of it for the first time, thus opening the way to its genetic manipulation. Transformation was accomplished using two different antibiotic resistance genes [hygromicine phophotransferase (hpt) and Shble] and it is credited by PCR amplification of both hpt/Shble and GUS genes and by the ß-glucuronidase activity of transformed cells. Nevertheless, the single 35S promoter seems unable to direct gene expression to a convenient level in S. almeriensis as suggested by the low GUS enzymatic activity. Temperature was critical for the transformation efficiency.


Subject(s)
Metabolic Engineering/methods , Molecular Biology/methods , Scenedesmus/genetics , Transformation, Genetic , Agrobacterium/genetics , Biotechnology/methods , Drug Resistance, Microbial , Gene Expression , Scenedesmus/radiation effects , Selection, Genetic , Temperature
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