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J Vet Med Sci ; 71(11): 1525-8, 2009 Nov.
Article in English | MEDLINE | ID: mdl-19959906

ABSTRACT

The immunohistolocalization and gene expression of carbonic anhydrase (CA) isoenzymes CA-II and CA-VI in the canine lower airways and lung were examined using specific canine CA-II and CA-VI antisera and the RT-PCR method. Laryngeal, tracheal and bronchial epithelia, serous acinar and bronchiolar secretory cells and pulmonary great alveolar cells showed immunopositive reactions to anti-CA-II and anti-CA-VI antisera. However, all mucous cells showed immunonegative reactions. The physiological roles of CA-II and CA-VI in the lower airways and lung may involve the maintenance of pH balance and the protection of mucosal surfaces against the acidic milieu.


Subject(s)
Carbonic Anhydrase II/metabolism , Carbonic Anhydrase IV/metabolism , Dogs/metabolism , Gene Expression Regulation, Enzymologic/physiology , Lung/enzymology , Animals , Carbonic Anhydrase II/genetics , Carbonic Anhydrase IV/genetics , Isoenzymes
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